HiLoad 16/600 and 26/600 Superdex 30, 75, and 0 prep grade Instruction for Use 2917 AF 3 CleaningInPlace (CIP) Regular cleaning Wash the column with onehalf to one CV 05 M NaOH at a flow rate of 08 mL/min for 16/600 or 22 mL/min for 26/600 to remove most of the nonspecifically bound proteins from the chromatography resin After cleaning, immediatelyHiLoad Superdex 75 pg prepacked columns are for highresolution size exclusion chromatography of recombinant proteins from samples up to 5 mL or 13 mL Prepacked format – for convenience and reproducibility;Superdex 75 16/60 a 10/300 GLor column (GE Healthcare) in mM Tris pH 75 containing 0 mM NaCl and 1 mM DTTSOD1 eluted from columnboth s as a single peak (~115 mL corresponding to ~32 kDa, ie dimer, on 10/300 GL columnthe and the fractions containing ) pure protein (≥ 90 %), as judged by SDSPAGE, were combined Matrix assisted laser desorption ionization timeof
Functional Characterization Of A Guanylyl Cyclase Activating Protein From Vertebrate Rods Journal Of Biological Chemistry
